Environmental effects of light. temperature, and pH of culture media on mycelial growth and fruit-body formation of Flammulina velutipes in synthetic media were investigated.
Illumination inhibited mycelial growth, but illumination during the latter part of vegetative growth induced primordia formation- The optimum light intensity and exposure time were 100¡1000 lux and 4¡8 hours per day, respectively. High intensity of light was injurous, and in darkness primordia developed into very poor fruit-bodies.
The optimum temperature was 25¡É for mycelial growth and 15¡É for fruit-body formation. The optimum pH range for mycelial growth was found to be from 5.0 to 7.0 and for fruit-body formation from 5.0 to 6.0. In low temperature treatments, a temperature of 15¡É was more effective than 5¡É or 10¡É, it took about 12 hours for primordium formation, but at 5¡É or 10¡É about 48 hours. The most excellent fruit-body formation were produced from the mycelium growth for 7 to 10 days.
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